Coding
Part:BBa_K197021:Design
Designed by: Gabriela Guzman Lopez Aguado Group: iGEM09_Berkeley_Wetlab (2009-10-21)
EILD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
Source
Wobble Osc0028F/Osc0029R (136bp, EcoRI/BamHI) Sub into pBca9495AK-Bca1144#5 (EcoRI/BamHI, 3171+910, L) Product is pBca9495AK-M10053 {<EILD>}
Osc0028F Forward construction of Leucine zipper EILD TCCGGgaattcATGagatctGTTAAAGAACTGGAAGACAAAAACGAAGAACTGCTGTCTGAAATCTACCACCTGGACAACGAAG Osc0029R Reverse construction of Leucine zipper EILD CTGGTggatccGCAACCACCACGTTCACCAACCAGTTTTTTCAGACGAGCAACTTCGTTGTCCAGGTGGTAG