Part:BBa_K5439005

IpfF coding sequence

Long-chain fatty acid CoA ligase from Sphingomonas spp. This enzyme catalyzes the conversion of ibuprofen into isobutylcatechol.

Sequence and Features IpfF coding sequence

Usage and Biology

Ibuprofen is an anti-inflammatory treatment drug widely used in the world that can be bought without any necessary prescription. This makes ibuprofen a drug that everyone can consume easily, bringing problems because its disposal makes it an emerging contaminant in water bodies 10. An example of it is Sphingomonas Ibu-2; an organism that has been grown in an environment rich in ibuprofen. The described organism has the ability to metabolize ibuprofen to isobutylcatechol due to the adaptation, which one particular gene is in charge of this degradation which is IpfF (Murdoch et al., 2013).

The gene ipfF participates in the lower ibuprofen degradation pathway, this gene encodes a CoA ligase enzyme which attaches CoA to ibuprofen (Jan-Roblero et al., 2023). Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1592
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 463
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1460
  

References

[1]. Murdoch, R. W., & Hay, A. G. (2013). Genetic and chemical characterization of ibuprofen degradation by Sphingomonas Ibu-2. Microbiology (Reading, England), 159(Pt 3), 621–632. https://doi.org/10.1099/mic.0.062273-0 [2]. Jan-Roblero, J., & Cruz-Maya, J. A. (2023). Ibuprofen: toxicology and biodegradation of an emerging contaminant. Molecules, 28(5), 2097