Coding
ECFP-RIFMO

Part:BBa_K5439003

Designed by: Osvaldo Sanchez, Diego Cota Barocio   Group: iGEM24_TecMonterreyGDL   (2024-09-30)
Revision as of 23:00, 1 October 2024 by Osvaldosan21 (Talk | contribs)


FRET-based system for the detection of rifampicin

FRET-based sensor system for the detection of rifampicin that consists of rifampicin monooxygenase (K4447003), an enzyme that catalyzes the hydroxylation of rifampicin, flanked by two fluorescent proteins: ECFP (BBa_K1159302) as energy donor and mVenus (BBa_K1907000) as an energy acceptor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1947
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2178
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2833


[edit]
Categories
Parameters
None