Part:BBa_K5292315
fedA
This signal peptide was selected through model prediction from a library of approximately 200 signal peptides in Escherichia coli, aiming to enhance the secretion expression of the PET-degrading enzyme ICCG. This signal peptide originates from Escherichia coli and was initially used to express F107 fimbrial protein. We constructed the signal peptide at the N-terminus of the ICCG-GFP fusion protein and characterized its secretion efficiency through fluorescence intensity. Although the secretion efficiency of this signal peptide in the experiment was not significantly improved, it provided valuable reference data for further optimization of PET-degrading enzyme expression.
In projects, this signal peptide can be used to guide the secretion expression of target proteins, especially in studies of recombinant protein secretion and applications in synthetic biology. Combining model predictions and experimental data, this signal peptide lays the foundation for subsequent optimization of signal peptides.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 9
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 9
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 9
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 9
- 1000COMPATIBLE WITH RFC[1000]
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