Part:BBa_K5292313
nfaA
This signal peptide was selected through model prediction from a library of approximately 200 signal peptides in Escherichia coli, aiming to enhance the secretion expression of the PET-degrading enzyme ICCG. This signal peptide originates from Escherichia coli and was initially used to express Non-adhesin 1. We constructed the signal peptide at the N-terminus of the ICCG-GFP fusion protein and characterized its secretion efficiency through fluorescence intensity. Although the secretion efficiency of this signal peptide in the experiment was not significantly improved, it provided valuable reference data for further optimization of PET-degrading enzyme expression.
In projects, this signal peptide can be used to guide the secretion expression of target proteins, especially in studies of recombinant protein secretion and applications in synthetic biology. Combining model predictions and experimental data, this signal peptide lays the foundation for subsequent optimization of signal peptides.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 72
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 68
- 1000COMPATIBLE WITH RFC[1000]
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