Signalling

Part:BBa_K212004:Design

Designed by: Elias Scheer   Group: iGEM09_Brown   (2009-10-20)
Revision as of 04:21, 22 October 2009 by Escheer (Talk | contribs) (→‎References)

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secretion signal peptide + GFPmut3b


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 760


Design Notes

We designed the signal peptide by taking the verbatim signal peptide from the beta-lactamase gene of ATCC 12228, a gene that we know is targeted for secretion by the cell through the Sec pathway. We searched for the sequence in the signal peptide database http://proline.bic.nus.edu.sg/spdb/. We then had the sequence synthesized by GeneArt(TM) and made into a biobrick standard part.

Source

This part was synthesized based on a known sequence, which may be found here: .

References

see 1 above