Part:BBa_K1680009
NanoLuc Luciferase
This part contains the protein coding region for the NanoLuc(tm) luciferase, which is originally designed by Promega Corporation (http://bit.ly/1Vgmab3, free for research use). Nanoluciferase is a small and monomeric enzyme (19.1kDa) and is about 150 times brighter than other luciferases. The new substrate called furimazine produces a high intensity luminescence.
After looking at the bibliography, Nanoluciferase gene was chosen because it allowed us to have a stronger signal. Indeed, this protein isn’t secreted but unfused. Furthermore, its intracellular half-life is superior to 6 hours and the Nanoluciferase has a low autoluminescence. This gives us maximum sensitivity. NLuc is used in biomedical research for several applications, including studying protein-protein interactions, monitoring protein stability, BRET-based sensors, molecular imaging or investigating genetic regulation and cell signaling. The emission pic of nanoluciferase is located at 460nm. We codon-optimized the part for yeast. The NanoLuc is smaller than other luciferases while yielding equal or stronger luminescence readings.
RLU measurement of the NanoLuc under pSUC2 promoter part:BBa_K950003 control, normalized to OD600.
RLU measurement of the NanoLuc under pFET3 promoter part:BBa_K950000 control, normalized to OD600.
MilkClear iGEM UCopenhagen 2024
Author: Kate Escobar, 26-09-2024, MilkClear's Wiki: https://2024.igem.wiki/ucopenhagen/
This part was used in biosensor devices, specifically in the reporter modules BBa_K5477030 and BBa_K5477031 to build these devices BBa_K5477041, BBa_K5477042, BBa_K5477043, BBa_K5477044, BBa_K5477045 and BBa_K5477046.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
//cds/reporter
//chassis/eukaryote/yeast
//function/reporter/light
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