Coding

Part:BBa_K5333000:Experience

Designed by: Michelle Vera Castellanos   Group: iGEM24_UiOslo-Norway   (2024-10-01)
Revision as of 21:09, 1 October 2024 by Michelvc (Talk | contribs) (→‎Applications of BBa_K5333000)

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Applications of BBa_K5333000

The original sequence build for the gBlock is encoded here

pET28-His-GR TCCTTTCGGGCTTTGTTACTTCTGATGAAACAGAAGTTTTTTGATATTGCCATTTGAATATTTTGGTATCTGATTGGTGATGATTTCAGCTAACATCTCGGGGAATTCAATACTCATGGTCTTATCCAAGAATGTCTGGAAGCAATAGTTAAGCAGATTTTCGACCACTTCATGCATAGAATCCAAGAGTTTTGTCAGTTGGTAAAAGCGCTGCCAGTTCTGGCTGGAGTTGCCTTCCCGTTTGACAATGGCTTTGCCCAGCTCTTTGATGTAGGTCATGCGAATTTCATCAAACAGCTCCTGGCTCTTCAGACCGTCCTTCGGGACTGAAGAGAGAAGCAGTAAGGTTTTCATACAGAGATATTCTTCATACGATACCTGAAGACGATGCAACTCCGAGGAAACATACAGCATGTGTTTACATTGGTCGTACATGCACGGCAGGGTCATGCGCTGCTCATTAATAATCAGATCAGGAGCAAAGCACAGCAGGTTTGCACTTGACTGACGATAGCTGCGCCACCCCAGCGCAAAGGCCATAAGAAACATCCAGGAGTACTGCAGCAAGGTCATTTGGTCATCCAGGTGTAAGTTGCGGAAACCCGGGATCGCTTTTGCCCATTTCACCGCTGCAATCACTTGACGCCCTCCCAGCATGTTCAGCGTAGTCATGATCCGCCAAGTAGAGTCCGGAACCGAGCTATCGTAACCGGCATATAACACTTCAGGTTCAATAACTTCCAACAGACTCACCAGCGTCGGCGTGAGTTGTGGTAACGTCGCGGGAACGCTGCCACGCGGTACCAGGCCATGATGGTGGTGATGGTGACCTTTTTTCATGGTATATCTCCTTCT

The original sequence is functional, however the modified sequence in the parts registry is the subsitute that fits the guidelines, as the original sequence includes illegal restriction enzyme cleavage sites.

Even thought the sequence used is directly derived from the literature (He et al.,2014) includes specific mutations to improve the solubility of the protein, we still encounter challenges to obtain the protein in the soluble fraction according to our laboratory results.

User Reviews

UNIQf48a6b28e574ae8e-partinfo-00000000-QINU UNIQf48a6b28e574ae8e-partinfo-00000001-QINU