Coding

Part:BBa_K5401010

Designed by: Ong Jie Hao   Group: iGEM24_NTU-Singapore   (2024-10-01)
Revision as of 21:03, 1 October 2024 by Registry (Talk | contribs)

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RNAPAnc302 Blast250

Ancestral sequence reconstruction has been a long-standing technique used in evolutionary biology to infer sequences of ancient proteins based on existing sequences. By using ancestral reconstruction, we are able to generate functional distant homologs through in silico methods. A programme called Molecular Evolutionary Genetics Analysis version X (MEGAX) was utilised for this purpose.

The ancestral sequence (RNAPAnc302 Blast250) was inferred after a series of workflow using a larger dataset of ~250 sequences. The ancestral sequence was subsequently cloned into stable T7-expressing plasmid (plasmid 1c) for downstream testing.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 647
    Illegal PstI site found at 2027
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 647
    Illegal PstI site found at 2027
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 647
    Illegal BamHI site found at 1491
    Illegal BamHI site found at 1621
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 647
    Illegal PstI site found at 2027
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 647
    Illegal PstI site found at 2027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 508


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