Composite

Part:BBa_K5175043:Design

Designed by: Yuhan Zou   Group: iGEM24_HUST-China   (2024-10-01)
Revision as of 20:31, 1 October 2024 by Yuhanzou (Talk | contribs)


T7 promoter-rhlA-rhlB-T7 terminator-T7 promoter-phaZ-T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 827
    Illegal PstI site found at 1811
    Illegal PstI site found at 1925
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2310
    Illegal NheI site found at 2366
    Illegal NheI site found at 3351
    Illegal PstI site found at 827
    Illegal PstI site found at 1811
    Illegal PstI site found at 1925
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 176
    Illegal BamHI site found at 736
    Illegal XhoI site found at 912
    Illegal XhoI site found at 2159
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 827
    Illegal PstI site found at 1811
    Illegal PstI site found at 1925
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 827
    Illegal PstI site found at 1811
    Illegal PstI site found at 1925
    Illegal NgoMIV site found at 1052
    Illegal NgoMIV site found at 1773
    Illegal NgoMIV site found at 1886
    Illegal NgoMIV site found at 2858
    Illegal NgoMIV site found at 3059
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nothing


Source

Pseudomonas aeruginosa, Pseudomonas putida

References