Composite

Part:BBa_K5175040:Design

Designed by: Yuhan Zou   Group: iGEM24_HUST-China   (2024-10-01)
Revision as of 20:28, 1 October 2024 by Yuhanzou (Talk | contribs)


T7 promoter-tphA2-tphA3-tphB-tphA1-T7 terminator-T7 promoter-tpaK-T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 912
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 2938
    Illegal PstI site found at 4853
    Illegal PstI site found at 5142
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3813
    Illegal NheI site found at 3878
    Illegal NheI site found at 3934
    Illegal NheI site found at 5409
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 2938
    Illegal PstI site found at 4853
    Illegal PstI site found at 5142
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 426
    Illegal BglII site found at 2027
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 912
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 2938
    Illegal PstI site found at 4853
    Illegal PstI site found at 5142
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 912
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 2938
    Illegal PstI site found at 4853
    Illegal PstI site found at 5142
    Illegal NgoMIV site found at 477
    Illegal NgoMIV site found at 1516
    Illegal NgoMIV site found at 2074
    Illegal NgoMIV site found at 2749
    Illegal NgoMIV site found at 3253
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nothing


Source

Comamonas sp.E6, Rhodococcus erythropolis

References