Part:BBa_K5106007
2 input AND gate for the detection of a dimerised miRNA trigger
This AND gate is designed for the detection of multiple miRNAs in combination. The toehold switch will be activated only if two specific miRNAs are present. It can be used to detect multiple disease-specific miRNAs to increase the accuracy of the test.
Usage and Biology
A 2-input AND gate is a single toehold switch that gets activated by two trigger RNAs. The input for the respective toehold switch is a combination of two input RNA molecules that are partially complimentary to each other. These partially complementary inputs hybridize to form an input trigger RNA that can anneal to the trigger binding region of the toehold switch. Once the trigger RNA is bound to the trigger binding region, the loop in the toehold switch opens up and the RBS is available for the ribosome to bind, allowing gene expression (Figure 1). The absence of any one trigger RNA will not lead to the unfolding of the loop structure, thus causing no gene translation.
ADD FIGURE
For experimental data of this part, see composite part BBa_K5106010, where the part is tested in a cell-free (PURExpress) system, under a T7 promoter and terminator, and regulating the LacZ reporter gene. For more details on the use of this part, take a look at miRADAR (WageningenUR 2024).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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