Composite

Part:BBa_K5175037:Design

Designed by: Xihong Zeng   Group: iGEM24_HUST-China   (2024-10-01)
Revision as of 20:12, 1 October 2024 by Yuhanzou (Talk | contribs)


T7 promoter- tphA2-tphA3 -tphA1-T7 terminator- T7 promoter - tphB-tpaK-T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 912
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 1951
    Illegal PstI site found at 4853
    Illegal PstI site found at 5142
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2826
    Illegal NheI site found at 2891
    Illegal NheI site found at 2947
    Illegal NheI site found at 5409
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 1951
    Illegal PstI site found at 4853
    Illegal PstI site found at 5142
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 426
    Illegal BglII site found at 3189
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 912
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 1951
    Illegal PstI site found at 4853
    Illegal PstI site found at 5142
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 912
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 1951
    Illegal PstI site found at 4853
    Illegal PstI site found at 5142
    Illegal NgoMIV site found at 477
    Illegal NgoMIV site found at 1516
    Illegal NgoMIV site found at 2266
    Illegal NgoMIV site found at 3236
    Illegal NgoMIV site found at 3911
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nothing.


Source

Comamonas sp.E6, Rhodococcus erythropolis

References