Composite

Part:BBa_K5175034:Design

Designed by: Xihong Zeng   Group: iGEM24_HUST-China   (2024-10-01)
Revision as of 19:50, 1 October 2024 by Andre-zeng (Talk | contribs) (Created page with " __NOTOC__ <partinfo>BBa_K5175035 short</partinfo> <partinfo>BBa_K5175035 SequenceAndFeatures</partinfo> ===Design Notes=== Nothing. ===Source=== <i>E.coli, Ideonella s...")

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T7 promoter- tphA2-tphA3 -tphA1-T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 912
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 1951
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2826
    Illegal NheI site found at 2891
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 1951
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 426
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 912
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 1951
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 912
    Illegal PstI site found at 949
    Illegal PstI site found at 982
    Illegal PstI site found at 1951
    Illegal NgoMIV site found at 477
    Illegal NgoMIV site found at 1516
    Illegal NgoMIV site found at 2266
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nothing.


Source

E.coli, Ideonella sakaiensis

References