Composite

Part:BBa_K5175033:Design

Designed by: Xihong Zeng   Group: iGEM24_HUST-China   (2024-10-01)
Revision as of 19:41, 1 October 2024 by Yuhanzou (Talk | contribs)


T7 promoter-lac operator-pelB-FAST-PETase-G4S-MHETase-Terminator-T7 promoter-fucO-aldA-T7 termina7 t


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
    Illegal PstI site found at 3117
    Illegal PstI site found at 4865
    Illegal PstI site found at 5578
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2820
    Illegal NheI site found at 2876
    Illegal NheI site found at 5636
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
    Illegal PstI site found at 3117
    Illegal PstI site found at 4865
    Illegal PstI site found at 5578
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1528
    Illegal BamHI site found at 4403
    Illegal XhoI site found at 2786
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
    Illegal PstI site found at 3117
    Illegal PstI site found at 4865
    Illegal PstI site found at 5578
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
    Illegal PstI site found at 3117
    Illegal PstI site found at 4865
    Illegal PstI site found at 5578
    Illegal NgoMIV site found at 166
    Illegal NgoMIV site found at 1327
    Illegal NgoMIV site found at 1715
    Illegal NgoMIV site found at 2078
    Illegal AgeI site found at 3833
    Illegal AgeI site found at 4034
    Illegal AgeI site found at 4675
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nothing.


Source

Ideonella sakaiensis, E.coli

References