Coding

Part:BBa_K5333000

Designed by: Michelle Vera Castellanos   Group: iGEM24_UiOslo-Norway   (2024-10-01)
Revision as of 19:30, 1 October 2024 by Michelvc (Talk | contribs)


pET28-His-GR

pET28-His-GR is a coding sequence that is used for expressing the ligand binding domain of the glucocorticoid receptor using Escherichia coli vector pET-28a(+).

This coding sequence will allow to express the LBD of Glucocorticoid Receptor from the vector including a his-tag for purification and attachment to an electrode for later ligand-binding measurements.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
directionReverse complement sequence