Part:BBa_K5401006
Antisense Oligonucleotide System (ASO)
This composite part entails an antisense oligonucleotide (ASO) targeting the T7RNAP mRNA, coupled with a Hfq scaffold sequence, under the lac promoter. The various elements work hand-in-hand to regulate the expression of T7RNAP, thereby allowing the stable expression of T7RNAP in the bacterial host cell.
The antisense oligonucleotide is a short RNA (sRNA) sequence complementary to the T7RNAP mRNA sequence. It binds to +4 to +27 of the mRNA sequence, thereby inhibiting translation and regulating the translation of T7RNAP. [BBa_K5401005]
Hfq is an RNA-binding protein that functions primarily as a riboregulator, facilitating the interaction between sRNAs and their target mRNAs. One of the key functions of the Hfq protein is RNA-RNA Interaction, through recognising its cognate Hfq scaffold sequence. [BBa_K5401004]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 141
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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