Composite
Delay test

Part:BBa_K257013:Experience

Designed by: Soufiane Boumahdi   Group: iGEM09_Paris   (2009-10-17)
Revision as of 04:00, 22 October 2009 by Benoît dH (Talk | contribs) (→‎Applications of BBa_K257013)

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Applications of BBa_K257013


PbadLacIYFP trans-1.jpg
x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar without glucose neither arabinose


PbadLacIYFP fluo.jpg
x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction on LB agar without glucose neither arabinose


PbadLacIYFP glucose trans1.jpg
x1000 observation on white light of E. coli with the pBad-LacI-YFP construction plated on LB agar with 1% glucose


PbadLacIYFP glucose fluo1.jpg
x1000 observation after fluorescence excitation of E. coli with the pBad-LacI-YFP construction plated on LB agar with 1% glucose


Conclusion :
In presence of glucose pBad is repressed and then no fluorescence is normally observed. Accordingly bacteria with our construction are less fluorescent in presence of glucose than in the control.