T7

Part:BBa_K5286002:Design

Designed by: Mara Valverde Rascón   Group: iGEM24_TUM-Straubing   (2024-10-01)
Revision as of 18:56, 1 October 2024 by Maravalverde (Talk | contribs) (→‎References)

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pT7max (T7 promoter variant)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

According to Deich et al., this promoter was created with the reasoning that a higher plasmid concentration usually yields higher translation in bacterial TxTl. It was also considered that increasing the promoter strength should increase protein abundance and increased DNA template protection from endogenous nucleases.


Source

This is a synthetic variant of the T7 promoter.

References

Deich, C., Cash, B., Sato, W., Sharon, J., Aufdembrink, L., Gaut, N. J., Heili, J., Stokes, K., Engelhart, A. E., & Adamala, K. P. (2023). T7Max transcription system. Journal of Biological Engineering, 17. https://doi.org/10.1186/s13036-023-00323-1