Composite
Part:BBa_K197032
Designed by: Jennifer Brophy Group: iGEM09_Berkeley_Wetlab (2009-10-21)
{Pbad.rbs.prepro.StrepTag}{type IIIs Needle Complex scFV}{upaG_short}(dblterm)
This is a cell surface display device that exposes a Type III Needle scFv to the extracellular environment via fusion to the autotransporter upaG_short. The Type III needle scFv will bind to the needle complex present on the surface of pathogenic E.coli. This device will enable engineered E.coli to bind preferentially to pathogenic E.coli. It can be used in projects that require pathogen detection.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1249
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1023
Illegal AgeI site found at 2383 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1005
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Categories
Parameters
None |