Composite

Part:BBa_K197032

Designed by: Jennifer Brophy   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 03:59, 22 October 2009 by Registry (Talk | contribs)

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{Pbad.rbs.prepro.StrepTag}{type IIIs Needle Complex scFV}{upaG_short}(dblterm)

This is a cell surface display device that exposes a Type III Needle scFv to the extracellular environment via fusion to the autotransporter upaG_short. The Type III needle scFv will bind to the needle complex present on the surface of pathogenic E.coli. This device will enable engineered E.coli to bind preferentially to pathogenic E.coli. It can be used in projects that require pathogen detection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1023
    Illegal AgeI site found at 2383
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1005


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