Composite
Part:BBa_K197034
Designed by: Jennifer Brophy Group: iGEM09_Berkeley_Wetlab (2009-10-21)
{Pbad.rbs.prepro.StrepTag}{mgfp-5}{GFP-LVA}{cl02365 AtD}(dblterm)
This is a cell surface display device that exposes mgfp-5 (a mussel foot adhesion protein) to the extracellular environment via fusion to the putative autotransporter {cl02365 AtD}. When expressed in the correct environment mgfp-5 should form an underwater stable bio-glue. This device will enable engineered E.coli to bind to surfaces.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2412
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1249
Illegal PstI site found at 2412 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2412
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2412
Illegal AgeI site found at 1023 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2337
Illegal SapI site found at 1005
[edit]
Categories
Parameters
None |