Composite

Part:BBa_K197033

Designed by: Jennifer Brophy   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 03:57, 22 October 2009 by Registry (Talk | contribs)

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{Pbad.rbs.prepro.StrepTag}{INP repeats}{VtaA11 AtD}(dblterm)

This is a cell surface display device that exposes a streptavidin tag to the extracellular environment via fusion to the autotransporter VtaA11 AtD. The streptavidin tag will bind to streptavidin present in the extracellular environment. This device will enable engineered E.coli to be preferentially purified.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1503
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1005


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