![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K197033
Designed by: Jennifer Brophy Group: iGEM09_Berkeley_Wetlab (2009-10-21)
{Pbad.rbs.prepro.StrepTag}{INP repeats}{VtaA11 AtD}(dblterm)
This is a cell surface display device that exposes a streptavidin tag to the extracellular environment via fusion to the autotransporter VtaA11 AtD. The streptavidin tag will bind to streptavidin present in the extracellular environment. This device will enable engineered E.coli to be preferentially purified.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1249
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1503
Illegal AgeI site found at 1023 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1005
[edit]
Categories
Parameters
None |