Generator
placIQ RBS

Part:BBa_K193602

Designed by: Kazuaki Amikura   Group: iGEM09_Tokyo_Tech   (2009-10-14)
Revision as of 03:57, 22 October 2009 by Ami (Talk | contribs)

placIQ RBS melA on pSB4A5

This composite part contains pLacIQ(BBa_I14032) and RBS(B0030) and melA(BBa_K193600) on low copy vector(BBa_I52001 derived).

We measured darkness of the cells expressing melA(BBa_K193602) in E. Coli strain JM109

Blackened E.coli
BBa K193602-2.gif


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1384
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1236
    Illegal BsaI.rc site found at 541


[edit]
Categories
Parameters
None