Plasmid

Part:BBa_K5120015

Designed by: Ananya Anuj Dharna   Group: iGEM24_Thailand-RIS   (2024-10-01)
Revision as of 18:10, 1 October 2024 by Registry (Talk | contribs)

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Hydroxyisoflavanone Dehydratase (HID) in pEAQ DEST 1

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 10226
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 10226
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5173
    Illegal NotI site found at 10232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 10226
    Illegal BglII site found at 644
    Illegal BglII site found at 1657
    Illegal BglII site found at 1913
    Illegal BglII site found at 1933
    Illegal BglII site found at 8791
    Illegal BglII site found at 9775
    Illegal BamHI site found at 1146
    Illegal XhoI site found at 714
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 10226
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 10226
    Illegal XbaI site found at 10241
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2397
    Illegal NgoMIV site found at 3353
    Illegal NgoMIV site found at 3636
    Illegal NgoMIV site found at 4168
    Illegal NgoMIV site found at 5045
    Illegal NgoMIV site found at 5169
    Illegal NgoMIV site found at 6196
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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