Composite

Part:BBa_K5175030

Designed by: Xihong Zeng   Group: iGEM24_HUST-China   (2024-10-01)
Revision as of 17:25, 1 October 2024 by Andre-zeng (Talk | contribs)


T7 promoter-lac operator-pelB-FAST-PETase-G4S-MHETase-T7 terminator

It is a composite component consisting of the T7 promoter, lac operator, target genes PETase-MHETase. It is responsible for enabling E.coli to degrade PET.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2820
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1528
    Illegal XhoI site found at 2786
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1645
    Illegal PstI site found at 1988
    Illegal NgoMIV site found at 166
    Illegal NgoMIV site found at 1327
    Illegal NgoMIV site found at 1715
    Illegal NgoMIV site found at 2078
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None