Composite

Part:BBa_K5129002

Designed by: Ansal' Diassova, Adil Ispambetov, Anastassiya Tyazhelova, Rauan Muratuly, Tomiris Kudassova, Makpal Akishova, Nuriya Nurlankyzy, Yernur Kenzhegazin, Artyom German, Abylaikhan Seraliyev, Temirlan Karat   Group: iGEM24_NU-Kazakhstan   (2024-10-01)
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L-lactate inducible production of anticancer peptide PNC27 (ALPaGA promoter)

The present composite part consists of the ALPaGA modified lactate inducible promoter, RBS B0033, NSP4 - PNC-27 and B1006 terminator. The composite parts release the NSP4 - PNC-27 in response to the high lactate concentration, which is then exported and targeted to the cancer cells, inducing their death via necrosis [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 166
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None