RNA

Part:BBa_K5401004

Designed by: Ong Jie Hao   Group: iGEM24_NTU-Singapore   (2024-10-01)
Revision as of 16:24, 1 October 2024 by Registry (Talk | contribs)

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Hfq scaffold sequence

Hfq is an RNA-binding protein that functions primarily as a riboregulator, facilitating the interaction between sRNAs and their target mRNAs. One of the key functions of the Hfq protein is RNA-RNA Interaction, through recognising its cognate Hfq scaffold sequence.

One of the key functions of the Hfq protein is to enhance the base-pairing between sRNAs and mRNAs, which can lead to the regulation of translation.

In our design: the Hfq protein binds to sRNAs (an antisense oligonucleotide targeted against T7RNAP mRNA), thereby helping them to recognize and bind to complementary regions on mRNAs. This binding blocks ribosome access to the mRNA's start codon or Shine-Dalgarno sequence, thus inhibiting translation. This helps to knockdown the expression of T7RNAP, thereby regulating the expression of T7RNAP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 80
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None