Plasmid

Part:BBa_K5401003

Designed by: Ong Jie Hao   Group: iGEM24_NTU-Singapore   (2024-10-01)
Revision as of 15:54, 1 October 2024 by Registry (Talk | contribs)

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p15A_T7Promoter_eCFP

T7 RNA Polymerase is a RNA polymerase derived from the T7 bacteriophage. It shows very high transcriptional activity, and is very specific to its cognate promoter: 5'-TAATACGACTCACTATAGG-3'. T7 RNA Polymerase is widely utilised in the field of biotechnology, such as in areas of in vivo protein expression and in vitro transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2948
    Illegal suffix found in sequence at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2948
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 2954
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2948
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2948
    Illegal suffix found in sequence at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2948
    Illegal XbaI site found at 2963
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2188


[edit]
Categories
Parameters
None