Coding

Part:BBa_K5059000

Designed by: Isaac Chang   Group: iGEM24_Cornell   (2024-09-16)
Revision as of 15:40, 1 October 2024 by Icyveins (Talk | contribs)


Alpha-Amyrin Synthase (AAS)

This sequence resembles the MdOSC1 variant of the alpha-amyrin synthase from Malus domestica. Alpha-amyrin synthase (AAS) converts 2,3-oxidosqualene to alpha-amyrin, the precursor for ursolic acid in the mevalonate pathway [1]. Since S. cerevisiae doesn't contain AAS, we integrated this sequence into its genome to successfully produce ursolic acid. We codon-optimized the sequence using Benchling to be compatible with iGEM Assembly Standards. We also attached a 6xHis tag at the end of the sequence to enable Ni-NTA affinity chromatography for convenient enzyme isolation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None