Composite

Part:BBa_K5117037

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-09-28)
Revision as of 15:07, 1 October 2024 by Jesa98 (Talk | contribs)


PcotYZ-BsRBS-BsEglS-L3-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
  • ribosome binding site of Bacillus subtilis (BBa_K5117000)
  • eglS gene of Bacillus subtilis without signal peptide encoding an endoglucanase (EC 3.2.1.4)
    • addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117025)
  • cotY gene of Bacillus subtilis (BBa_K5117022)
  • bidirectional terminator B0014 (BBa_B0014)

    • </u>


    Biosafety level: S1

    Target organism: Bacillus subtilis

    Main purpose of use: Immobilization of BsEglS on the spore crust of B. subtilis (spore surface display)

    Application: Degradation of cellulose


    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 1669
      Illegal AgeI site found at 726
    • 1000
      COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None