Composite

Part:BBa_K5102115

Designed by: Natalia Kuźmierkiewicz   Group: iGEM24_Munich   (2024-10-01)
Revision as of 15:06, 1 October 2024 by Nataliak-2024 (Talk | contribs)

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pcDNA_Zeo_NLuc_tape1.1

The part consists of a CMV enhancer, CMV promoter, Program recording tape 1.1, T2A, NLuc and bovine growth hormone polyadenylation signal. In state one of the RNA recording state of the tape, the Nanoluciferase is in the same open reading frame as first start codon and NLuc is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 718
    Illegal SpeI site found at 15
    Illegal SpeI site found at 701
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 718
    Illegal NheI site found at 661
    Illegal SpeI site found at 15
    Illegal SpeI site found at 701
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 718
    Illegal BglII site found at 1115
    Illegal BamHI site found at 695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 718
    Illegal SpeI site found at 15
    Illegal SpeI site found at 701
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 718
    Illegal SpeI site found at 15
    Illegal SpeI site found at 701
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None