Plasmid

Part:BBa_K243035:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-20)
Revision as of 03:24, 22 October 2009 by Timo (Talk | contribs) (→‎Design Notes)

pJS419


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2266
    Illegal XbaI site found at 1382
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2267
    Illegal PstI site found at 2281
    Illegal NotI site found at 2274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1494
    Illegal BamHI site found at 2365
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2267
    Illegal XbaI site found at 1382
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal suffix found in sequence at 2257
    Illegal XbaI site found at 1382
    Illegal NgoMIV site found at 1390
    Illegal NgoMIV site found at 2498
    Illegal AgeI site found at 3832
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2114


Design Notes

Commented GenBank file This vector is only a expression vector. It has the additional restriction sites AgeI and NgoMIV within the vector. It has a weak RBS for a low expression level,an ampicilin resistance and lacI operon.

Source

References