Plasmid_Backbone

Part:BBa_K5226052:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-09-30)
Revision as of 14:40, 1 October 2024 by Admin (Talk | contribs) (→‎Source)

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321(C+cysNC) backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 2528
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 2981
    Illegal NgoMIV site found at 3866
    Illegal NgoMIV site found at 4977
    Illegal NgoMIV site found at 5101
    Illegal AgeI site found at 2037
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 2103
    Illegal SapI.rc site found at 2171


Design Notes

none


Source

The pSEVA321 backbone is directly obtained from the laboratory.CysNC and its RBS are obtained by PCR amplification.Sequence source is from Halomonas sp. TD01 strain, found through NCBI(https://www.ncbi.nlm.nih.gov/nuccore/OV350343.1?from=2677331&to=2679235)

References