Plasmid

Part:BBa_K5302023

Designed by: Xinyuan Shi   Group: iGEM24_USTC   (2024-10-01)
Revision as of 14:36, 1 October 2024 by Hagibis (Talk | contribs)


8IJZ

This work is derived from pBBRMCS-OmpA-mCherry and PUC19-ZVEGF-V114-V107-peptide, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and V107(approximately 2kda), we succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express V107. The plasmid uses lac promotor and has kanamycin resistence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid
Parameters
biologyEscherichia coli Nissle 1917