Intermediate

Part:BBa_K218018:Design

Designed by: Emily Hicks   Group: iGEM09_Calgary   (2009-10-18)
Revision as of 03:19, 22 October 2009 by Emily Hicks (Talk | contribs)

LuxO D47E with strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 136
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 824
    Illegal SapI.rc site found at 786


Design Notes

The plasmid containing LuxO D47E (pJAF822) was obtained from Bassler lab (Freeman and Bassler, 1999).

>gi|156972381:c2983472-2982069 Vibrio harveyi ATCC BAA-1116 chromosome I, complete sequence Nucleotide number 141 has been mutated from T to G, thus bases 139-141 have been changed from GAT (Aspartic acid, D) to GAG (Glutamine, E).



Source

Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.


References