Part:BBa_K5143014

mRuby2 : reporter Red Fluorescent Protein

Description

mRuby2 is a red fluorescent protein that was engineered to monitor biochemical processes in living cells using changes in Fluorescence Resonance Energy Transfer (FRET). Therefore, mRuby2 can be used as a reporter system. Here, mRuby2 was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pADH1 (BBa_J63005). The excitation wavelength is 559nm and the emission wavelength is 600nm.

mRuby2 Red Fluorescent Protein used as a transcriptional reporter.

Construction

The mRuby2 used was synthesized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene mRuby2 was synthesized in fusion with the secretion signal AGA2 (BBa_K5143020) in 5' and a 6XHis tag in 3'. See the composite part here: BBa_K5143021.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Lam, A. et al. Improving FRET dynamic range with bright green and red fluorescent proteins. Nat Methods 9, 1005–1012 (2012).

2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).