Part:BBa_K5291050
PE-binding peptide
Plastic-binding peptides are adsorbed to the plastic surface by hydrogen bonding and other intermolecular forces. Considering that PE-binding peptides（PEBP） has not been extensively studied and we did not find the sequence of PEBP in the paper, we decided to predict PEBP sequence by modeling method. We performed machine learning training; then performed special processing and tried to introduce neural networks. Finally, several predictors were given.
Usage and Biology
In degradation module we constructed pAB1-pS-PEBP-PEase to depolymerize PE microplastics.
Fig.1 The map of plasmid pAB1-pS-PEBP-GFP.
Moreover, PEBP-GFP proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.
Fig.2 The SDS-PAGE result of PEBP-GFP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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