Coding

Part:BBa_K5233032

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-10-01)
Revision as of 14:07, 1 October 2024 by Qwerty (Talk | contribs)


tpiA-tpiB-tphC

This composite part is a transporter system that can transport TPA into the bacteria cell. It use tripartite tricarboxylate transporter(TTT) family protein to transport.It also use tphC, a kind of solute-binding protein (SBP) to binds TPA and enables tpiA, tpiB to transport TPA into the cell. We use this transporter element to transport TPA into the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 437
    Illegal PstI site found at 1989
    Illegal PstI site found at 2849
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 437
    Illegal PstI site found at 1989
    Illegal PstI site found at 2849
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2278
    Illegal BamHI site found at 1519
    Illegal BamHI site found at 2028
    Illegal BamHI site found at 3017
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 437
    Illegal PstI site found at 1989
    Illegal PstI site found at 2849
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 437
    Illegal PstI site found at 1989
    Illegal PstI site found at 2849
    Illegal NgoMIV site found at 1473
    Illegal NgoMIV site found at 2776
    Illegal AgeI site found at 718
    Illegal AgeI site found at 1635
    Illegal AgeI site found at 2111
    Illegal AgeI site found at 2570
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None