Regulatory

Part:BBa_K5292057

Designed by: 2024 TJUSLS-China   Group: iGEM24_TJUSLS-China   (2024-10-01)
Revision as of 13:24, 1 October 2024 by Registry (Talk | contribs)

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Mtac511

The tac promoter is a strong promoter capable of initiating robust gene transcription in E. coli. To generate a dataset for training a predictive model of tac promoter strength, which can then be used to design promoters that enhance ICCG expression, we designed a pair of degenerate primers to randomly mutate the 16 bp sequence between the -35 and -10 regions of the tac promoter, creating the Mtac promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None