Coding

Part:BBa_K212005:Design

Designed by: Elias Scheer   Group: iGEM09_Brown   (2009-10-20)
Revision as of 03:01, 22 October 2009 by Escheer (Talk | contribs) (→‎References)

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TrgEnvZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 348
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The genes coding for the two domains were ligated together using a shared NdeI site.

Source

Both parts come from genomic sources.

References

1. Baumgartner, James W., Changhoon Kim, Renée E. Brisette, Masoyuri Inouye, Changkyu Park, and Gerald L. Hazelbauer. "Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of osmosensor EnvZ." Journal of Bacteriology 176: 1157-163. Journal of Bacteriology. Web. 25 July 2009.