Coding

Part:BBa_K5059000

Designed by: Isaac Chang   Group: iGEM24_Cornell   (2024-09-16)
Revision as of 12:50, 1 October 2024 by Icyveins (Talk | contribs)


Alpha-Amyrin Synthase (AAS)

This sequence resembles the MdOSC1 variant of the alpha-amyrin synthase from ""Malus domestica"". Alpha-amyrin synthase (AAS) converts 2,3-oxidosqualene to alpha-amyrin, the precursor for ursolic acid in the mevalonate pathway [1]. Since "S. cerevisiae" doesn't contain AAS, we integrated this sequence into its genome to successfully produce ursolic acid. We also added a 6x-His tag at the end of the sequence to enable Ni-NTA chromatography.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Ursolic acid has gained traction recently as a potential therapeutic agent. Preliminary studies have been done on ursolic acid, determining its therapeutic potential for cancer, liver disease, and obesity, among other health benefits [2]. There is particular interest in ursolic acid's properties in fighting cancer, as it is an antioxidant and anti-inflammatory agent. Clinical trials are currently underway to test its use in cancer-treating drugs. However, the current method for ursolic acid extraction from fruits, such as apples and loquats, is inefficient, environmentally taxing, and expensive [REF]. By engineering "S. cerevisiae" to produce ursolic acid, the traditional method for its extraction can be bypassed by utilizing the pathway shown below.

Figure 1: Metabolic Pathway for Producing Ursolic Acid in "S. cerevisiae"

Within this pathway, AAS is the first enzyme required to catalyze ursolic acid production. Specifically, it catalyzes the conversion of 2,3-oxidosqualene to alpha-amyrin, which is the direct precursor for ursolic acid through the action of Cytochrome P450, which is activated via Cytochrome P450 reductase donating an electron from NADPH to it.

Functionality

Catalytic Efficiency

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This data comes from Dr. Ryan Summers in [2].

Substrate Product Km (µM) kcat (min^-1)</th> kcat/Km (min^-1/µM)</th> 
 </tr>
1. 2,3-oxidosqualene alpha-amyrin 50.07 43.4 0.87
2. Theobromine 7-methylxanthine 25 46 1.8
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