Coding

Part:BBa_K5233022:Experience

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-09-28)
Revision as of 12:48, 1 October 2024 by Lambertbai (Talk | contribs) (→‎Applications of BBa_K5233022)


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Applications of BBa_K5233022

This is the plasmid map of the secretion of TurboPETase, containing our basic part turboPETase(ompA) turbopetase-ompa-2.png

After the construction of the plasmid, we successfully express the engineering E.coli that can secrete the turboPETase.After that, we test the enzyme activity and compare the enzyme activity with other TurboPETases which have different siganl peptide, the process is done by mixing the bacteria with BHET substrate solution,and the detection is done by HPLC. the details procedures are shown in : https://2024.igem.wiki/hust-ueve-upsaclay/protocol

The results of the enzyme activity is shown as follows: hplc2.png

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UNIQa4e5ba3df245d217-partinfo-00000000-QINU UNIQa4e5ba3df245d217-partinfo-00000001-QINU