Composite

Part:BBa_K231001

Designed by: Daniel R Tarjan   Group: iGEM09_Virginia   (2009-10-21)
Revision as of 02:59, 22 October 2009 by ThaddeusWebb (Talk | contribs)

Phytochelatin Synthase, HA-tag

Codes for phytochelatin synthase (PCS) which catalyzes the conversion of glutathione (GSH) to phytochelatin (PC). HA Tag appended after a TG scar/linker to enhance protein detection ability. The precursors for PC are naturally present in E. coli and have been shown to be upregulated in the presence of PCS and heavy metals (Li, Dhankher, Carriera. 2004). PC is a cysteine rich molecule capable of binding heavy metals. The PCS gene is endogenous to A. thaliana


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
    Illegal NgoMIV site found at 1449
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None