Coding

Part:BBa_K5233022:Experience

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-09-28)
Revision as of 12:35, 1 October 2024 by Lambertbai (Talk | contribs)


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Please enter how you used this part and how it worked out.

Applications of BBa_K5233022

screenshot-2024-10-01-104806.png This is the plasmid map of the secretion of TurboPETase, containing our basic part turboPETase(ompA)

After the construction of the plasmid, we successfully express the engineering E.coli that can secrete the turboPETase.After that, we test the enzyme activity and compare the enzyme activity with other TurboPETases which have different siganl peptide, the process is done by mixing the bacteria with BHET substrate solution,and the detection is done by HPLC. the details procedures are shown in : https://2024.igem.wiki/hust-ueve-upsaclay/protocol

The results of the enzyme activity is shown as follows: 20241001111333.png

User Reviews

UNIQ2af9f393c15b8765-partinfo-00000000-QINU UNIQ2af9f393c15b8765-partinfo-00000001-QINU