Composite

Part:BBa_K231001

Designed by: Daniel R Tarjan   Group: iGEM09_Virginia   (2009-10-21)
Revision as of 02:55, 22 October 2009 by ThaddeusWebb (Talk | contribs)

Phytochelatin Synthase, HA-tag

Codes for phytochelatin synthase (PCS) which catalyzes the conversion of glutathione (GSH) to phytochelatin (PC). HA Tag appended after a TG scar/linker to enhance protein detection ability.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 100
    Illegal PstI site found at 1363
    Illegal PstI site found at 1396
    Illegal PstI site found at 1507
    Illegal NgoMIV site found at 1449
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None