Part:BBa_K5226085
Mmp1-SOD
Contents
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 665
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 665
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 665
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 665
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 665
- 1000COMPATIBLE WITH RFC[1000]
Introduction
In order to produce multiple classes of high value-added bioproducts while absorbing assimilated CDE, based on the current research of related literature, we iGEM24-SCUT-China-A chose to introduce the synthesis pathway of PHA particle-associated protein.
Usage and Biology
The SOD gene produces an antioxidant enzyme that converts harmful superoxide radicals into less active hydrogen peroxide, protecting cells from oxidative damage. To test the ability of Halomonas TD to produce phaP proteins, we chose to use the inducible promoter Mmp1. In this experiment given, we added 50 mg/L of IPTG to the flask fermentation system for induced fermentation.Experimental characterisation
growth conditions
shake flask studies
experimental design
Data Processing and Analysis
The fermentation broth was centrifuged to obtain the supernatant and precipitate after cell disruption, and they were subjected to SDS-PAGE, and the following results were obtained after shooting: from left to right, the protein results obtained by fermentation under two carbon sources, sodium acetate and glucose, were phaP, TD80 control, SOD, Amalase, Meta and sodium acetate and glucose. It can be seen that compared with the control group, the TD80 after the introduction of protein synthesis pathway genes can produce phaP and SOD proteins, and the protein produced with glucose as the carbon source is more than that with sodium acetate as the carbon source.
References
[1]Programmable bacteria induce durable tumor regression and systemic antitumor immunity
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