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DsbA

Part:BBa_K243002

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-08)
Revision as of 02:48, 22 October 2009 by Timo (Talk | contribs) (→‎Usage and Biology)


DsbA signal sequence (enables periplasm export)

DsbA encodes a periplasmic disulfide bond isomerase. Lokalized in the periplasm, it catalyzes the forming of disulfide bonds and therefore enables the correct folding of proteins in this compartment.


Usage and Biology

The signal sequence of the DsbA protein linked to the coding sequence of another protein enables export of the latter into the periplasm. The accumulation of the expressed protein in the periplasm can be used for the purification of the protein and to prevent interaction with cytoplasmic components. Freiburg 09 DsbA grafik.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None