Part:BBa_K5302035

pBBR-OmpA-VEGFR1D2-l2masking#29

This work is derived from pBBR-OmpA-VEGFR1D2 and pUC19-VEGFR-masking#29, and it has undergone codon optimization. This composite part combines OmpA(21.4kda) and VEGFR1D2(approximately 12kda), and it adds a fragment as a mask. We succeeded in transferring this plasmid into Escherichia coli Nissle 1917 and let it express VEGFR1D2. The plasmid uses lac promotor and has kanamycin resistence.

Figure 1. Colony PCR results of pBBR-OmpA-VEGFR1D2-l2masking#29

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2367
  Illegal XbaI site found at 1296
  Illegal PstI site found at 123
  Illegal PstI site found at 1284
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2367
  Illegal PstI site found at 123
  Illegal PstI site found at 1284
  Illegal NotI site found at 5299
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2367
  Illegal BglII site found at 6045
  Illegal BamHI site found at 1302
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2367
  Illegal XbaI site found at 1296
  Illegal PstI site found at 123
  Illegal PstI site found at 1284
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2367
  Illegal XbaI site found at 1296
  Illegal PstI site found at 123
  Illegal PstI site found at 1284
  Illegal NgoMIV site found at 574
  Illegal NgoMIV site found at 857
  Illegal NgoMIV site found at 3025
  Illegal AgeI site found at 1955
  Illegal AgeI site found at 2865
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 5621
  Illegal SapI.rc site found at 423
  Illegal SapI.rc site found at 633