Composite

Part:BBa_K208045

Designed by: USU iGEM 2009   Group: iGEM09_Utah_State   (2009-10-20)
Revision as of 02:37, 22 October 2009 by Cdmiller (Talk | contribs)

Lac Promoter/RBS/GFP/Term

GFP expression composite part using K208000 GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 230
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 749
    Illegal XhoI site found at 650
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

GFP characterization-1.jpg

GFP activity is not observed in cells without the BBa K208045 BioBrick. GFP activity is also not seen in BBa K208045 containing cells with the lac repressor and no IPTG. Cells express the GFP when in cells that lack the lac repressor. These results demonstrate the functionality of this BBa K208045 BioBrick.


GFP gel.jpg

The bright band at 27 kD in the GFP+ sample corresponds to the GFP protein. The absence of this band in the GFP- sample further reinforces the functionality of the GFP construct.



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