Composite

Part:BBa_K5301020

Designed by: Jiaxin Guan   Group: iGEM24_BNU-China   (2024-09-24)
Revision as of 09:37, 1 October 2024 by Guanjiaxin (Talk | contribs)

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SnCSdT expression pathway (T7)

This pathway expresses SnCSdT using T7 expression system.

Usage and Biology

We used this pathway to express SnCSdT by using T7 expression system in E.coli, and the expression of SnCSdT can be induced by IPTG. For more information on SnCSdT, go to BBa_K5301006.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1081
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1223
    Illegal PstI site found at 1081
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 263
    Illegal BglII site found at 635
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1081
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1081
    Illegal AgeI site found at 160
    Illegal AgeI site found at 920
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None